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首页> 外文OA文献 >Multigene Assessment of Biodiversity of Diatom(Bacillariophyceae) Assemblages from the Littoral Zone of the Bohai and Yellow Seas in Yantai Region of Northeast China with some Remarks on Ubiquitous Taxa
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Multigene Assessment of Biodiversity of Diatom(Bacillariophyceae) Assemblages from the Littoral Zone of the Bohai and Yellow Seas in Yantai Region of Northeast China with some Remarks on Ubiquitous Taxa

机译：东北烟台地区渤海和黄海沿岸带硅藻（细菌科）群落生物多样性的多基因评估及泛在生物分类

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Diatoms are important contributors to the benthic microeukaryote flora. This manuscript lays the foundation for future metagenomic and environmental sequencing projects off coastal China by curating diatom DNA sequences from the Yantai region of the Bohai and Yellow Seas (Northeast China). These studies are based on cultures established from samples collected in different seasons from marine littoral and supralittoral zones in 2013 and 2014. Thirty-six diatom strains were cultured successfully and identification of these clones was determined by light and scanning electron microscopy(LM and SEM) and DNA sequencing of the nuclear-encoded small subunit ribosomal RNA (SSU) and chloroplast-encoded rbcL and psbC genes. The strains primarily represent raphid pennate genera, such as Amphora, Amphora (Oxyamphora), Caloneis, Diploneis, Halamphora, Navicula, Nitzschia, Parlibellus, Pleurosigma, Surirella and Tryblionella. When the DNA markers from these strains were analysed in a multi-gene phylogeny, we found that some clones-particularly within the genera Amphora, Navicula and Nitzschia-show greater than expected genetic diversity despite their very similar morphology and morphometrics. We also compared the molecular and morphological identities of several seemingly ubiquitous marine littoral taxa in the genera Amphora and Nitzschia from the Indian Ocean and Atlantic Ocean, the Red Sea and Adriatic Sea to their Yellow Sea counterparts.
机译：硅藻是底栖微真核生物菌群的重要贡献者。该手稿通过策划来自渤海和黄海烟台地区（中国东北）的硅藻DNA序列，为中国沿海未来的宏基因组学和环境测序项目奠定了基础。这些研究基于2013年和2014年从海洋沿海和上滨带不同季节采集的样品建立的培养物。成功培养了36株硅藻菌株，并通过光学和扫描电子显微镜（LM和SEM）鉴定了这些克隆。核编码的小亚基核糖体RNA（SSU）和叶绿体编码的rbcL和psbC基因的DNA和DNA测序。菌株主要代表蚜虫的半翅类，例如安芙兰，安芙兰（Oxyamphora），卡洛尼斯，Diploneis，Halamphora，Navicula，Nitzschia，Parlibellus，Pleurosigma，Surrella和Tryblionella。在多基因系统发育分析中分析了这些菌株的DNA标记后，我们发现一些克隆，特别是Amphora，Navicula和Nitzschia属内的克隆，尽管其形态和形态非常相似，但其遗传多样性却超出了预期。我们还比较了印度洋和大西洋，红海和亚得里亚海的安菲拉和尼兹菌属中几种看似普遍存在的海洋沿海分类单元的分子和形态特征，以及它们的黄海对应物。

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